protogel83. Pour Stacking Gel Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mLShop National Diagnostics ProtoGel (30%), 37.5:1 Acrylamide to Bisacrylamide Stabilized Solution, 1 liter at